The headless doll owned by Morticia Addams in The Addams Family is named Anne Boleyn, Wednesday Addams's doll is named Marie Antoinette.

Music & song
Anne is referenced in Tori Amos' song "Talula" on her Boys for Pele album with the lyrics 'Ran into the henchman who severed Anne Boleyn, he did it right quickly a merciful man. she said 1+1 is 2, but henry said that it was 3.'

Anne Boleyn is referenced in Roger Waters' song "Watching TV" on his Amused to Death album.

She is also referenced in a song titled "Old Age", written by Courtney Love and performed by her band Hole: "Someone please tell Anne Boleyn, Chokers are back in again." The song appeared on their outtakes album, My Body, the Hand Grenade.

The headless doll owned by Morticia Addams in The Addams Family is named Anne Boleyn, Wednesday Addams's doll is named Marie Antoinette.

The song "Transylvania" by McFly mentions Anne Boleyn and is portrayed by Dougie Poynter in the music video.

Anne Boleyn is mentioned in Blues Traveler's song "Hook".

Tori Amos' song "Talula" includes a verse about Anne Boleyn.

In Ugly Betty,Betty Suarez wears a replica of Anne Boleyn's necklace.

In a dream sequence at the start of Kevin & Perry Go Large, the teenage character Kevin is reading a book on Anne Boleyn for his homework, but instead his mind wanders to a sexual fantasy in which Anne (played by Natasha Little and speaking in 20th century teenage slang) convinces her executioner (Kevin) that to kill her would be a waste of her beautiful body and in return gives him oral sex.

The ghost of Anne haunting the Tower of London is the subject of the comically macabre song "With Her Head Tucked Underneath Her Arm", originally performed by Stanley Holloway and later recorded by The Kingston Trio.

In his 1973 album The Six Wives of Henry VIII, Rick Wakeman titled the fifth track "Anne Boleyn".

In British TV series The Office, the main character speaks of a club in town that had a bowling alley inside called the "Anne Boleyn" alley. Also there was a washroom in this club with a sign stating "Mind your Head," and "Don't get your Hampton Court (pron. caught)".

The Dutch symphonic rock band Kayak issued a single called Anne, about Anne Boleyn, from their album Periscope Life (1980).

In "Gossip Girl" (2009) , season two finale, Blair tells Jenny that "Anne Boleyn thought only with her heart and she got her head chopped off. So her daughter Elizabeth made a vow never to marry a man. She married a country.". Blair says this in order to make Jenny understand that if she wanted to be Queen Bee of Constance Billard School she needed to be cold.